FastQCFastQC Report
Wed 8 May 2024
EGAF00007947590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520066
Total Bases531.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG49289614.002464726513649Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA487101.3837808722904628No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACT84840.24101820818132388TruSeq Adapter, Index 11 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAG83170.2362739789538037TruSeq Adapter, Index 11 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC59600.16931500716179754Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG57020.1619855991336526Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGCA40550.1151967036981693TruSeq Adapter, Index 11 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGC39140.11119109698511334TruSeq Adapter, Index 11 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGCT38450.10923090646595832TruSeq Adapter, Index 11 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGCC36560.10386168895696841TruSeq Adapter, Index 11 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGAC35940.10210035834555375TruSeq Adapter, Index 11 (97% over 37bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTT35570.10104924169035466TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)