FastQCFastQC Report
Fri 10 May 2024
EGAF00007947591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520066
Total Bases531.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG50120814.238596662676212Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA296600.8425978376541804No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138790.39428237993264903No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCT61650.1751387616027654Illumina Single End Sequencing Primer (100% over 33bp)
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC59920.17022408102575348No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG58510.16621847431269754Illumina Single End PCR Primer 1 (100% over 32bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.12391813108049679No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG42090.11957162166845736Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCTTCGGTGT41340.11744097979981057Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT39710.1128103848052849Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCTTCG39700.11278197624703629Illumina Single End Sequencing Primer (100% over 33bp)
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG39370.11184449382483168No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG36460.10357760337448219Illumina Single End PCR Primer 1 (96% over 30bp)
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35620.10119128448159778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)