FastQCFastQC Report
Wed 1 May 2024
EGAF00007947592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25728614
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA687940.267383233313695No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG527160.20489249829003614Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT519920.20207851071962135No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA288770.1122369047940165No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)