FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25728614
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC891390.3464586160762488No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG754110.29310168048694735No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC745880.28990290732334045No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT606900.23588522879623444No Hit
CCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCC535440.20811070506946078No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG533580.20738777456103932Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA377290.14664217823781722No Hit
CCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCA367990.14302752569570984No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA347820.13518800507481668No Hit
CTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC332220.1291247169396688No Hit
CCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCG314480.12222967004751986No Hit
CCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGG312910.12161945451084151No Hit
CTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAG309570.1203212889742137No Hit
CCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCC298360.11596427230786703No Hit
GCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCG293550.11409475846619642No Hit
CCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTAT291840.11343012880522829No Hit
TCCCCCGCCGACCCCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCC275800.10719582485088391No Hit
CTCGTCCAGCCGCGGCGCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGAC270260.10504258021827372No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA263580.10244624914501807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)