FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669683
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1919895.231759800505929Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA315650.8601560407261335No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGA91550.24947658966728187TruSeq Adapter, Index 25 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGT73990.20162504499707468TruSeq Adapter, Index 25 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAA67970.18522035827072802TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTA57720.15728879033965604TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACT53350.14538040479245756TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAA53150.14483539858892444TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAA50370.1372598123598142TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAT50010.1362788011934546TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTC48420.13194600187536634TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTT48260.13150999691253987TruSeq Adapter, Index 25 (97% over 40bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTA46110.1256511802245589TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAC45280.1233894044798965TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAC45090.12287164858654004TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACA44390.12096412687417415TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAT43790.1193291082635748TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGTCT41910.11420604995036356TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCCA39990.10897399039644569TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGATA38410.10466844138853411TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCCT37970.10346942774076126TruSeq Adapter, Index 25 (97% over 40bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCA37840.10311517370846474TruSeq Adapter, Index 25 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)