FastQCFastQC Report
Wed 8 May 2024
EGAF00007947598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8085895
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG5372436.644199559850826Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2650223.2775839903931474No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTGGA164710.20370039432864268TruSeq Adapter, Index 16 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93590.11574476294831926No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG88640.1096229916416179No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)