FastQCFastQC Report
Wed 8 May 2024
EGAF00007947602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7824454
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2512203.2107032644066917No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1235201.5786405032223334Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124330.15889926632580367No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110400.14109610715329146No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108730.13896177292370818No Hit
CTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104100.1330444271255221No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81980.10477408391690972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)