FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51562757
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG4966950.9632824714939118Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTAA2407780.4669610664922359TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTCA2361710.45802632314637487TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACACT2274290.4410722258315241TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACACA2133130.41369587743339636TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAAT2069500.40135557530409016TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTAT2027120.3931364647549781TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTAC2021580.39206204586771803TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTCT2021160.3919805917282507TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAAA1977870.3835849972102927TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCTC1938480.3759457625588174TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCAA1923540.37304832245490677TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCAT1903790.3692180385156674TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAAC1886980.3659579335526997TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATC1850500.3588830597246768TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCCA1838590.3565732530554951TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTTA1808400.3507182519352098TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATT1795140.34814662838916854TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACACC1772000.3436588931813712TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGCA1769680.34320895603002766TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCTA1731700.33584317456104995TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATA1713120.33223979858175545TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCCT1681780.3261617682700714TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGCT1651800.32034749421951975TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTTT1593410.3090234294492825TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCAC1512540.29333962883326814TruSeq Adapter, Index 20 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1432460.2778090395748234No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCTT1409610.2733775465109439TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTGC1378310.267307273736352TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTCC1314050.2548447903978447TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCCC1313420.25472260918864364TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGCC1265260.2453825345297188TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTTC1260640.24448653899557773TruSeq Adapter, Index 20 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACA1185430.2299004298780998TruSeq Adapter, Index 18 (97% over 34bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCG1181650.2291673426228935TruSeq Adapter, Index 20 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAA1164630.22586651059019205TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTGA1140090.22110726158417016TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGTC1131090.21936181573844085TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGTA1120270.21726340195501959TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTAG1102690.2138539644030283TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGAA1056710.20493667551562456TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAAG1052600.20413958857940817TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAGC1050360.2037051665022489TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATG1036740.20106372512237852TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAGT1019130.1976484694175682TruSeq Adapter, Index 20 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT992300.1924451014130218Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGTT978600.18978814495896718TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCGC965670.1872805210939361TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTGT950070.18425508162800527TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTTG947230.18370429649446401TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGAT920160.17845438326736485TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCGT870670.16885637050012667TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTCG867320.16820667676866075TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAGA858480.16649226107129997TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCAG847850.16443069558906634TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGAC828210.1606217448768304TruSeq Adapter, Index 20 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACTA825530.16010198989165766TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACACG810100.1571095199583684TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGGC759670.14732920506946515TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGGT737820.143091650432889TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCTG722140.1400506958927739TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCGA684380.13272758087780295TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACCCG633840.12292593276189634TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAGG607160.11775165552144545TruSeq Adapter, Index 20 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGCG601810.11671408493537302TruSeq Adapter, Index 20 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTA590350.1144915505584777TruSeq Adapter, Index 20 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACAT576230.11175313996495571TruSeq Adapter, Index 20 (97% over 37bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTC571180.11077375090707427TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACGGA561630.10892163892632815TruSeq Adapter, Index 20 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)