FastQCFastQC Report
Wed 15 May 2024
EGAF00007947607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51562757
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5029890.9754889561083788Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2323890.4506915718257657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1638820.3178301734331234No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1064970.2065386069251495Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTTAGCTGTGT814200.15790466751031174Illumina Single End Sequencing Primer (100% over 33bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT699740.13570647512118095No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTTA649790.1260192506773833Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)