FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768010
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG39248214.179211780304263Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGT194390.7022734744455403TruSeq Adapter, Index 3 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGA173500.6268040939158457TruSeq Adapter, Index 3 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112550.4066098027102503No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGC95430.3447603151722718TruSeq Adapter, Index 3 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTC88360.319218499933165TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG86830.3136910632548293Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGG79400.2868486746796435TruSeq Adapter, Index 3 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTG75640.2732649087250407TruSeq Adapter, Index 1 (97% over 36bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA73210.26448603870650755No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAA72340.26134298647765TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTA66090.23876358828183422TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA65530.23674047420348915No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC61260.2213142293561078No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGC60520.21864082860972323TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGT59940.2165454604571515TruSeq Adapter, Index 1 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA55580.20079407227575047No Hit
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT55060.19891546634585858Illumina PCR Primer Index 2 (96% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTT52440.1894501826221726TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCA51560.18627100335620173TruSeq Adapter, Index 1 (97% over 36bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC49080.17731149815210206No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCT48130.17387942962633804TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG46650.1685326281335689Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTC43950.15877832811297649TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGT43840.1583809307047301TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCG43730.15798353329648374TruSeq Adapter, Index 1 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCG43350.15661070588617815TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTT42920.155057243290306TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGT42540.15368441588000042TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTA42060.15195031809856177TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCT41390.14952980661197035TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAT41200.14884339290681753TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAT40510.14635062734599946TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGA40490.14627837327177284TruSeq Adapter, Index 1 (97% over 36bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG39970.14439976734188098No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAG39470.14259341548621574TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAA39240.1417624936326097TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAC39240.1417624936326097TruSeq Adapter, Index 1 (97% over 36bp)
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC38850.1403535391851908No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCT37900.1369214706594268TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGA37870.13681308954808688TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGC37700.1361989299171607TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGA36560.1320804476862439TruSeq Adapter, Index 1 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC35980.1299850795336722Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTCT35710.12900964953161295TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGG35580.12853999804914TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTG35320.12760069508419405TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAC34910.12611948656254857TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAC34880.1260111054512086TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC34650.1251801835976026RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAA34560.12485504026358288TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTA34440.1244215158182232TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAT34430.1243853887811099TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG34070.1230848154450309Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAA33400.12066430395843947TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAT33240.12008627136462657TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTC33210.11997789025328667TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG33110.11961661988215361Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT32890.1188218250656609Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTT32500.11741287061824199TruSeq Adapter, Index 1 (97% over 36bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG31820.11495623209453723No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTG31710.11455883468629087TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG31520.11387242098113808Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGACT30790.1112351472718668TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGC30550.11036809838114747TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGG30340.10960943060176806TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTCA29950.10820047615434916TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCA29320.10592447281621091TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTT29130.10523805911105812TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCG28920.10447939133167872TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAG28470.10285367466157998TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTA28450.10278142058735337TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGG27700.10007189280385546TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)