FastQCFastQC Report
Thu 9 May 2024
EGAF00007947609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768010
Total Bases417.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG42896915.49737898345743Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG701642.534817432017948No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93960.33944964071661593No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGT88800.3208080895661504Illumina Single End Sequencing Primer (100% over 33bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC85750.30978934324659235No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC76350.2758299283600854No Hit
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA70620.2551291360941615Illumina Single End PCR Primer 1 (100% over 25bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA66750.2411479727313124No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC59630.21542552230663908No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA56490.20408163265306123Illumina Single End PCR Primer 1 (100% over 26bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA56310.20343134598502172No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT55880.20187788338914958Illumina Single End PCR Primer 1 (96% over 26bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA55820.20166112116646978No Hit
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGTAGATC39890.14411075104497456Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTG38310.13840267918107232Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG36740.1327307343542834Illumina Single End PCR Primer 1 (100% over 32bp)
TGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG36460.13171917731511085No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT35550.12843161693780009No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT35240.12731167878728764Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG34570.12489116730069617No Hit
TCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCG30740.11105451208630027Illumina Single End Sequencing Primer (100% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGTAGATC29410.10624961615023068Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)