FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29918870
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2180310.7287407579230099Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAA1252840.41874576145422604TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAAA1233350.41223147799365417TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATAA970400.324343800417596TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAAT964780.3224653872288626TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAAC945400.3159878698627321TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATA882960.2951180977089041TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACAA882420.29493760960891907TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAACA855270.2858630690263369TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAACT745630.2492172999849259TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATT738080.246693808957357TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATAT718980.240309878013441TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATTA698570.23348809630845016TruSeq Adapter, Index 12 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA654190.21865464838745582No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATC644350.21536575412106138TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATCA639560.21376475782674945TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGAA636390.21270522583239274TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATAC628990.21023187038815305TruSeq Adapter, Index 12 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATA621390.2076916674994744TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAAG619410.2070298777995292TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACTA596080.1992321234057302TruSeq Adapter, Index 12 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAT586430.19600673421155276TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACAT573370.1916415960896919TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAACC568260.18993364388427772TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATCT566110.1892150338565594TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACCA519730.17371311149117596TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATTT496340.16589530286404533TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACAC490150.16382637445866102TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATGA471090.15745581300363282TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGTA467380.15621579290929102TruSeq Adapter, Index 12 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAA439190.14679364561562652TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATG435520.1455669950101725TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATAG429810.1436585004714416TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATTC421570.14090438576055847TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACCT416640.1392565962551393TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAGA404510.13520229874991935TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGCA400690.13392551256113616TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAGT393130.13139867916134532TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGAT386270.1291058118170907TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAGC380910.12731430030612786TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACTT373150.12472061946189814TruSeq Adapter, Index 12 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATG367420.12280544017872332TruSeq Adapter, Index 12 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGC351780.11757797002360049TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATGC344540.11515809253491191TruSeq Adapter, Index 12 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAAC341450.11412529951833074TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGCT336300.11240397782402878TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATCC334680.1118625135240736TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATTG332660.1111873543352406TruSeq Adapter, Index 12 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACA323150.10800875835217039TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCATGT321770.1075475109855419TruSeq Adapter, Index 12 (97% over 37bp)
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT315660.10550532155793317No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAACG314110.10498725386353161TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCACAG313050.10463296240800539TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAGTT308000.10294506443592288TruSeq Adapter, Index 12 (97% over 37bp)
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG300640.10048507848057096No Hit
CCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAG300540.1004516547583515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)