FastQCFastQC Report
Thu 2 May 2024
EGAF00007947621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29918870
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2179360.728423232561925Illumina Single End PCR Primer 1 (100% over 30bp)
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1726640.5771073573300061No Hit
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC740130.24737899526285587No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC626280.20932608751600576No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA460040.153762491698383No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412410.13784277280525636No Hit
CCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCG399470.13351774315005882No Hit
CTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC354450.11847038340685996No Hit
CTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGC352870.11794228859579256No Hit
CTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACA347970.11630452620703924No Hit
GGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACATGT342210.1143793198071986No Hit
CTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAG332420.11110713740191391No Hit
CCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCC330210.11036847314086394No Hit
TCCCCCGCCGACCCCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCC328730.109873802052016No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT319200.10668852132450189No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCC312600.10448255565801783No Hit
CCCGGCCCCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCCTCCGG304320.10171507145824693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)