FastQCFastQC Report
Wed 8 May 2024
EGAF00007947625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5066934
Total Bases765.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG71179514.047844317687975Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580881.1464131958300623No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT143360.28293244001204676Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTGT89610.1768525108083113Illumina Single End PCR Primer 1 (97% over 34bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80920.15970209992867482No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG79710.1573140680340419Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT79450.15680093721370755No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG74930.1478803552602027Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGAC66640.13151937641184983Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG62750.1238421499076167Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGT59740.11790167387220753Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT57230.11294798787590288Illumina Single End PCR Primer 1 (96% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTGTAGATC56960.11241512125478642Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTG56750.11200066943836252Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)