FastQCFastQC Report
Wed 8 May 2024
EGAF00007947627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12073495
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG402924333.372631537098414Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2266991.877658457637991No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGT974150.8068500463204731Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG669500.554520459899971Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGT622260.5153934299885824Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT526600.436162022678603Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG480730.39816970976506805Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTG472470.39132827735465164Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG456860.37839912966378003Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG405700.336025318269482Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAT365060.3023648082017676Illumina Single End PCR Primer 1 (97% over 35bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGA346110.28666927016576393Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT332090.27505705680086834Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGATC331460.27453525263397216Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT296130.24527280625866826No Hit
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGT235050.19468264988721162Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT232400.19248775934391824Illumina Single End PCR Primer 1 (96% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGATC219790.1820433934001712Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT218670.18111574154791135Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTG193520.16028498790118356Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGACGAGCGTCGTGTAGGGAAAGAG193200.16001994451482357Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG189850.15724527156386778Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGG175340.14522721051360854Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT170180.14095338590855425No Hit
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATG164290.1360749310783663Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT151860.12577965203944674No Hit
CGTATCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT146760.12155552306933493Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT136700.1132232216106438Illumina Single End PCR Primer 1 (100% over 31bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG136660.11319009118734881Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT127480.10558665904114757Illumina Single End PCR Primer 1 (100% over 31bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTA122930.10181807339134194Illumina Single End PCR Primer 1 (97% over 35bp)
TCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTG122640.10157787782245323Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)