FastQCFastQC Report
Wed 8 May 2024
EGAF00007947628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10998575
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG110295910.028199107611668Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCG471080.4283100310722071TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA357730.3252512257269692No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCT313560.2850914777596189TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCA310350.2821729178552676TruSeq Adapter, Index 7 (97% over 35bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACA255410.23222099226490706TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTA253290.23029346983586507TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCC248140.22561104506720187TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTG243560.22144686925351692TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTC238010.216400761007676TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAC229290.20847246120520158TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAA228870.20809059355416498TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGC223910.20358091843716117TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTA221330.20123516000936484TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAC221020.2009533053145521TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAC214450.19497980420190797TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGC209720.19067924708428138TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTT207140.18833348865648503TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAA206150.1874333720504702TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGC200680.18246000050006478TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAT199570.18145077885089658TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGA192970.17545000147746415TruSeq Adapter, Index 10 (96% over 33bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGG191830.17441350356750762TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAT190610.17330426896211554TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCC181820.16531232455113504TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTC180760.16434856333661407TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAC179470.16317568412271588TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG178810.16257560638537263Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGT174420.1585841802233471TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC173080.15736584057480174Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCA168740.15341987484742342TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTT168310.15302891510945738TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACT165630.15059223581236658TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGT159970.1454461146103018TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCT159120.1446732872212991TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCT152950.1390634695858327TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTCT146490.13318998142941244TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTA145480.1322716806495387TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACC142360.12943494952755244TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGT141670.12880759552942087TruSeq Adapter, Index 10 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACA139810.12711646736054444TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCC137920.12539806293087968TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAT137220.12476161684581866TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCG136380.12399788154374544TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAGC135390.12309776493773057TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAG134150.12197034615847961TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTT133170.1210793216393942TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAA130750.11887903660246896TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAA129150.11742430269375806TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGA128530.11686059330413257TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGT126710.11520583348297393TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTCC126270.11480578165807843TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTC124800.11346924487945029TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATG119290.10845950498132712TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAT119110.10829584741659715TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGA118890.10809582150414941TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT118750.1079685322871372Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCA118720.10794125602634887TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATA117450.10678656098630959TruSeq Adapter, Index 7 (97% over 35bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAT115410.1049317752527032TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATC114950.10451353925394881TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCGC111170.1010767303946193TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)