FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10998575
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG116177310.562941108279936Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG952450.8659758195948112No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG498680.45340419099747015Illumina Single End PCR Primer 1 (96% over 33bp)
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG423350.38491350015797504Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGT318070.2891920089647977Illumina Single End PCR Primer 1 (96% over 33bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA256310.23303928008855693No Hit
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAGATC183460.16680342680756371Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG169580.15418361014949666Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCAC165080.15009217103124722Illumina Single End PCR Primer 1 (96% over 33bp)
CGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG154590.14055457184226136Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTG145700.13247170656198642Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT133420.12130662381263028Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTT132080.12008828416408489Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT122120.11103256558235954Illumina Single End PCR Primer 1 (100% over 31bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG114350.10396801403818222Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)