FastQCFastQC Report
Wed 8 May 2024
EGAF00007947631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664340
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG97488114.62832028377904Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG948421.4231266712082518No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTGT234740.352232929292323Illumina Single End Sequencing Primer (100% over 33bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTGTAGATC151830.22782451075425325Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT129210.1938826650501025Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGC112480.16877890383743926Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG109480.16427733278914342Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAC102420.1536836355888205Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG99300.14900200169859282Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTG91370.1371028488942641Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT75530.11333455375926198Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)