FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11298341
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3422563.0292588973903336Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT482580.4271246548497695Illumina PCR Primer Index 2 (96% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA443570.392597461875155No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAA328950.29114893947704357TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAT249120.2204925484192768TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAA230290.20382638477631365TruSeq Adapter, Index 3 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCT206800.1830357217931376TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATA195060.1726448157300262TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATT184150.16298853079403428TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCT170040.15049997163300347TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAT157190.13912662044808172TruSeq Adapter, Index 3 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAA153750.13608192565616492TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTTA153330.13571018966412857TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCACA138400.12249586023293155TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAGA137690.12186744938925104TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCCAA133910.11852182546092387TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAG127020.11242358502013704TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC124020.10976832793416307RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCAAC122360.10829908567992416TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCA122000.10798045482960728TruSeq Adapter, Index 3 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTCA121180.10725468455944107TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTGA120960.10705996570646965TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTCT114520.1013600138285789TruSeq Adapter, Index 3 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCA113940.1008466641252906TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCATC113280.10026250756637632TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCTCTAG113140.10013859556903089TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)