FastQCFastQC Report
Wed 1 May 2024
EGAF00007947638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28225800
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2752940.9753275372177229No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1195370.42350261108631115Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAA1105260.3915779180749527TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAAC903400.32006178744269426TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCACT888010.3146093290535609TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAAA780060.2763641774546691TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAAT766850.2716840620992142TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCAA757470.26836086133962544TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCACA723560.2563470300221783TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATT720050.25510348688079704TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATA715780.25359068653501404TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTAA714960.2533001721828965TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCAT692470.24533228464737933TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTCT679590.24076908360436197TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCTA674120.23883114030426064TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTTA671200.23779662578208588TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTA670930.23770096861736426TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATC667950.2366451969474736TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAT666090.23598622536828007TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTAC610670.21635170659467579TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTAT604910.21431102041394753TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCAC599810.21250416285809437TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCACC589370.20880541915552436TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTTT584320.2070162758894345TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTCA573740.20326793217552733TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCTT569160.2016453032332122TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCCT547500.19397147290776523TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTGT525470.18616655683807015TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTGC523170.1853516995089599TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCCA517300.1832720418907524TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCA505440.1790702123589057TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTGA483340.17124049628354202TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCTC472310.1673327239617655TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTTC470890.16682963813248872TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAC467290.16555420926953354TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGCT427320.15139340603277854TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAGC425340.1506919201581532TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATG424750.15048289153894664TruSeq Adapter, Index 15 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCA421510.149335005562287TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTT398010.14100928937355184TruSeq Adapter, Index 15 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGAC386740.13701648846091166TruSeq Adapter, Index 15 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA366400.12981031538521495No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGAC360510.12772357205110219TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCT359460.1273515719660736TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCTG354810.12570414301808983TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCCC354400.12555888584203104TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTTG354070.1254419715295935TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGCA347950.12327374246256971TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAGT347470.12310368528084235TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGAC343530.12170779924749696TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGAA337590.11960334162362093TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTAG336050.11905774149891235TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGTA331510.11744928398840777TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTCC330460.11707728390337917TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAGA321100.11376116885969574TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCAAG317420.11245739713311935TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCCAG307880.10907751064628815TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGTT299130.10597750993771655TruSeq Adapter, Index 15 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCTG298940.10591019563661613TruSeq Adapter, Index 15 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCGTG284260.10070928016212118TruSeq Adapter, Index 15 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)