FastQCFastQC Report
Wed 8 May 2024
EGAF00007947640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10751635
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG219960.20458283786605477Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC142330.13237986594597007No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT137210.12761779952537453No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC125810.11701476101076719No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)