FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759821
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG69688318.53500472495898Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT220210.5856927763316392Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAA178960.4759801064997509TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAA166820.44369133530559035TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAT157490.4188763241654323TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAA140210.37291668938494676TruSeq Adapter, Index 18 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGAT131250.34908576764691723TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATA130460.34698460378831864TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCT127370.3387661274300026TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAC122510.3258399801479911TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTA117280.31192974346385105TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACA117060.31134460922474766TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACT116760.31054669889869757TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATT112810.30004087960570464TruSeq Adapter, Index 18 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110960.2951204325950624No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCA108810.28940207525837TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCA107660.28634341900851135TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAC104470.277858972541512TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAA103290.2747205252590482TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTA99960.26586372063989216TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATC96120.25565046846645095TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAT95640.25437381194477077TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG93480.2486288575972101Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAT91710.2439211866735145TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAT86710.2306226812393462TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTT85070.22626077145693904TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTA83980.22336169727229035TruSeq Adapter, Index 18 (97% over 38bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT82770.22014345895722165Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAC82620.21974450379419658TruSeq Adapter, Index 18 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATC82140.21846784727251645TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCT80600.2143719075987926TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCA78720.20937166955554534TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTT75640.20117979020809768TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACC74000.19681788042569046TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAA73740.19612635814311372TruSeq Adapter, Index 18 (97% over 38bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC73590.19572740298008867Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAC70110.18647164319790754TruSeq Adapter, Index 18 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCA70070.18636525515443422TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGAT70040.1862854641218292TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGA69430.18466304645886067TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTC68410.18195015135029036TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTC67860.18048731575253182TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAG67640.17990218151342843TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCA67100.17846594292653825TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC66260.17623179401359798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGA63670.16934316819869882TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGT61930.16471528830760826TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAG61850.16450251222066156TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATG61510.16359821385113812TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCT60860.16186940814469625TruSeq Adapter, Index 18 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTT59570.15843839374268082TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGC55930.14875708178660635TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC54530.14503350026503922RNA PCR Primer, Index 29 (96% over 29bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCT54480.14490051521069755TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTG53720.14287914238470395TruSeq Adapter, Index 18 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCT52630.13998006820005526TruSeq Adapter, Index 11 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCC51260.13633627771109316TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCC50520.13436809890683624TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCC50050.13311803939602443TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGC49690.13216054700476432TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTC48150.1280646073310405TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAT47100.12527192118986516TruSeq Adapter, Index 18 (97% over 38bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG46870.12466018993989342Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCT46240.1229845782551882TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGTA44850.11928759374448944TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAG44600.11862266847278101TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCG43080.11457992282079385TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAG42070.11189362472309188TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGT41890.1114148785274618TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGCA41680.11085634129922675TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGA41500.1103775951035967TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGCT41490.11035099809272836TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCG41430.11019141602751834TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACG38660.10282404401698911TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCC38350.10199953668007067TruSeq Adapter, Index 18 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)