FastQCFastQC Report
Thu 9 May 2024
EGAF00007947643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759821
Total Bases567.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG69262518.421754652681603Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233340.6206146516017651No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA148640.3953379695469545Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCAAGCTGTGT145500.3869865081342968Illumina Single End Sequencing Primer (100% over 33bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA102050.2714224959113745Illumina Single End PCR Primer 1 (100% over 26bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA79840.21235053477279905Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT79050.21024937091420043Illumina Single End PCR Primer 1 (100% over 31bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78550.2089195203707836No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT71240.18947710542602958Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG68420.18197674836115868Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG67200.17873191303522162Illumina Single End PCR Primer 1 (100% over 32bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG63410.16865164591612206Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG61360.16319925868811308Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT49560.13181478586347595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)