FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8081000
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG161768120.018326939735182Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA589800.7298601658210617No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTA372920.46147753990842716TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACA269710.33375819824279174TruSeq Adapter, Index 9 (97% over 35bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC214780.2657839376314813TruSeq Adapter, Index 9 (100% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG192410.238101720084148Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACT170380.21084024254423958TruSeq Adapter, Index 9 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC149530.1850389803242173Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTAC148920.18428412325207277TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACC140450.17380274718475436TruSeq Adapter, Index 9 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACG125170.15489419626283876TruSeq Adapter, Index 9 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG120890.14959782205172628Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTC115930.14345996782576415TruSeq Adapter, Index 9 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT115320.1427051107536196Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTCT113760.1407746566019057TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGC104140.12887018933300334TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAC103910.12858557109268656TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTT99850.12356144041579013TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTA97870.12111124860784556TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACT96520.11944066328424699TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAT91520.11325331023388195TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGCT89390.11061749783442643TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCT88890.10999876252938993TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTGC86740.10733820071773297TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCC85440.10572948892463804TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG83560.10340304417770078Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAA83040.10275955946046282TruSeq Adapter, Index 9 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG81540.10090335354535329Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)