FastQCFastQC Report
Wed 8 May 2024
EGAF00007947649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8081000
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG164008320.295545105803736Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG921441.140254918945675No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA477840.5913129563172874No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCATGTGT146150.18085632966217052Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT136530.16895186239326818Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG133660.1654003217423586Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT120880.14958544734562554No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTC115970.14350946665016706Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGTG108320.13404281648310853Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT106690.1320257393886895Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG88950.11007301076599431Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCAT86810.10742482366043807Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCATGTG81610.10098997648805841Illumina Single End Sequencing Primer (100% over 33bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG81150.10042074000742483Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)