FastQCFastQC Report
Thu 9 May 2024
EGAF00007947650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4962366
Total Bases749.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG4590909.251433691106218Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCG188680.38022185384955487TruSeq Adapter, Index 2 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA173490.3496114555032821No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAA128320.2585863275703566TruSeq Adapter, Index 2 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGT98790.19907842347783294TruSeq Adapter, Index 2 (97% over 36bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA79530.1602662923291027Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAC66890.1347945717828955TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAA64020.12901104029811586TruSeq Adapter, Index 2 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTA61320.1235700873333406TruSeq Adapter, Index 2 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC59690.12028536387682812Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAT58740.11837095450033311TruSeq Adapter, Index 2 (97% over 36bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC57060.11498547265558405Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTACA56730.11432046729322264TruSeq Adapter, Index 2 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG55000.11083422706023699Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTTA51310.10339825800837746TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTACC51080.10293476942248919TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTACT49810.10037550636128008TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)