FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40727266
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG26333836.465896826956172Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3990310.9797637779074099No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAAC2592990.6366717569502456TruSeq Adapter, Index 6 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACT2134310.5240494169188769TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTA2025200.4972590107079616TruSeq Adapter, Index 6 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACG1948170.4783453915124084TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAA1911810.46941771146631844TruSeq Adapter, Index 6 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAA1465070.3597270683477747TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTA1325870.3255484912736347TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAC1304960.32041433863986846TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAT1263500.3102344262440793TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGA1192450.29278911086248705TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGC1162390.2854083060719077TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTT1100890.2703078571490657TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTG995840.2445143261028128TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT932210.2288908860221553Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCA896240.22005896492045401TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTAG870600.21376342816628052TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC859150.21095204377332863Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTC857230.21048061512403018TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGT835850.2052310606854877TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTT789910.19395114810800215TruSeq Adapter, Index 6 (97% over 36bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGA780240.19157681735867071TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCT675230.16579310774261155TruSeq Adapter, Index 6 (97% over 36bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAC667180.16381654491612574TruSeq Adapter, Index 6 (97% over 36bp)
CCTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAG610510.1499020336891752No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTA592620.14550939903503465TruSeq Adapter, Index 9 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTG580180.14245493424478825TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT538130.13213015575364181Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG513660.12612189583263458Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA513610.12610961904489243TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTTC489160.12010626983898207TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGCC483130.11862568923727904TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTT482000.11834823383430647TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTGCT476430.1169805996798312TruSeq Adapter, Index 6 (97% over 36bp)
CTCCACCCGCCCTCCCTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGG465160.11421341172275104No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG450770.11068015221056086Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CCCGGCCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGC437960.10753483919102255No Hit
CAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT422110.10364309747676163Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCC421930.1035989010408899TruSeq Adapter, Index 6 (97% over 36bp)
CTTCCCCCGCCGCCCCTCCTCCTCCTCCCCGGAGGGGGCGGGCTCCGGCG415770.10208640079105728No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTCTC411730.10109443634149172TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)