FastQCFastQC Report
Sat 4 May 2024
EGAF00007947653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40727266
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG26314386.461121156524477Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2538390.62326550473582No Hit
CCTGCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCC1977700.485596062352921No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC1622180.39830319079115206No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1514130.3717730524803703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1096380.269200490894724No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCA1042940.2560790601559162Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT937390.2301627612322418Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG840790.2064440073144119Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCGCCGCGC687930.16891141182911715No Hit
CTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAG658300.16163618741312025No Hit
GGCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGC642700.15780582963757006No Hit
CTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGAC641850.15759712424595354No Hit
GCGGACCCGGCGGGGGGGACCGGCCCGCGGCCCCTCCGCCGCCTGCCGCC581320.14273484500530922No Hit
CCCGTCGCCGGGGCGGGGGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCG552020.1355406473884105No Hit
CCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCC538090.13212033432344808No Hit
CGCCGCCCCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCCCGACCC530070.13015113756960753No Hit
GCCGCCCCGACCCTTCTCCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCG521720.12810091401666884No Hit
TCCCCCGCCGACCCCACCCCCGGCCCCGCCCGCCCACCCCCGCACCCGCC516390.12679220844335587No Hit
CGGCCCGCGGCCCCTCCGCCGCCTGCCGCCGCCGCCGCCGCGCGCCGAGG509390.12507345815945514No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA503230.12356095790962252No Hit
CCCGGCCCCGCCCGCCCACCCCCGCACCCGCCGGAGCCCGCCCCCTCCGG494810.1214935468538448No Hit
CGGTCCCGCCGCCCCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGCCC490840.12051876990711824No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCATTG477360.11720894793183516Illumina Single End Sequencing Primer (100% over 33bp)
CCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCC463230.11373952771590413No Hit
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCAT459800.1128973400767928Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT457590.11235470605858984Illumina Single End PCR Primer 1 (100% over 31bp)
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCC451650.11089622367482264No Hit
CGTATGCCGTCTTCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT430740.10576207104105637Illumina Single End PCR Primer 1 (100% over 31bp)
CCCCCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGG425190.10439934760167796No Hit
CCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGA420520.10325269562656134No Hit
CCCGCCGCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGA415730.10207657936086355No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG409620.10057635589877306Illumina Single End PCR Primer 1 (96% over 30bp)
CCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGG409010.10042657908831887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)