FastQCFastQC Report
Wed 1 May 2024
EGAF00007947654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26341847
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG4932181.872374401081291Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430600.543090239647964No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA651190.24720741867493196No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA517990.19664148835121545No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA419390.15921055194041633No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCA363620.13803891579812153TruSeq Adapter, Index 6 (97% over 37bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC300770.11417954101699854No Hit
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG287860.10927859386625394No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCACGTTAA280540.10649974544305872TruSeq Adapter, Index 6 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)