FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13906289
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG268233119.288618264729003Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGC981740.70596835719436TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGT913640.6569977080154167TruSeq Adapter, Index 10 (96% over 33bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATC650770.4679681257882674TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGA587860.42272960097406287TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTT586780.4219529739386259TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTA580570.4174873684848632TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGT577900.41556737386947734TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTG482360.3468646451975793TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCT456500.3282687422935048TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGC442180.3179712430828958TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCA441700.3176260755115905TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGG415450.2987497239558303TruSeq Adapter, Index 10 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG400760.2881861580756735Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAA388320.2792405651860104TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAT376740.2709133975282694TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGA366310.2634131938434474TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTA354540.254949397355398TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAT347550.2499228945982641TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTC341600.24564425491229183TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGC341490.2455651540105344TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTT329710.2370941665314161TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTT325690.234203388121734TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGG325240.23387979352363525TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAG321540.23121912682815665TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCG317390.22823486553457936TruSeq Adapter, Index 9 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCG313950.22576116460689116TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGT306570.22045421319807174TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAA299930.21567939512834805TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGT298350.214543218539468TruSeq Adapter, Index 9 (97% over 39bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCA291030.20927941307706177TruSeq Adapter, Index 9 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTA290830.2091355932556845TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAT289240.20799222567573564TruSeq Adapter, Index 9 (97% over 39bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC270700.19466012823406734Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTA266700.19178373180652292TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTG262760.18895048132539172TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGA258380.1858008272372306TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTG253540.18232038755990185TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAC247880.17825028661492653TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAA247800.17819275868637563TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTT242720.17453973522339425TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTC240000.17258378565266408TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCT235850.16959952435908673TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGG226440.16283280176328854TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCC224220.1612364017460014TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAT220300.15841753324700789TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGG219390.15776315305974153TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAC213180.15329754760597883TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGC212300.15266474039191907TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCT211530.15211103407961676TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTC210440.15132721605311095TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGGC209530.15067283586584457TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAT206540.14852272953625514TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAG204950.14737936195630624TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGC204570.14710610429568952TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGT202960.1459483547336029TruSeq Adapter, Index 9 (97% over 39bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC194630.1399582591732417Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAA192690.13856320690588264TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAA192290.1382755672631282TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGT190210.13677984112080513TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATG188030.13521220506779344TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCA186570.13416232037173972TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGGT186530.13413355640746427TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCA185210.13318434558637463TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG184420.1326162572919346Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTC182790.13144412574771025TruSeq Adapter, Index 9 (97% over 39bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT180320.1296679509537016TruSeq Adapter, Index 9 (97% over 36bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA174520.1254971761337622TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATT173670.12488594189290904TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAC170610.12268549862583757TruSeq Adapter, Index 9 (97% over 39bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT169870.12215336528674184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCG169380.12180100672436767TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG167530.12047067337662837Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGA166790.11993854003753265TruSeq Adapter, Index 9 (97% over 39bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT163310.11743607514556903Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAC162060.11653720126196142TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG161190.11591158503897049Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG159010.1143439489859588Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATA156070.11222979761171367TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAC153440.11033856696060322TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAG152700.10980643362150751TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAG151510.10895070568431305TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCC150290.108073404773912TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTG150000.10786486603291502TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGC145340.10451386419482582TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGG141490.10174533263331433TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAG140520.10104780649963481TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGG140110.10075297586581151TruSeq Adapter, Index 9 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)