FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13906289
Sequences flagged as poor quality0
Sequence length151
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG273886919.695182517780268Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6961365.005907758712623No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGT1027090.7385795016916447Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTG647260.4654440879230972Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGT521210.3748016455001043Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT492580.35421383806995527Illumina Single End PCR Primer 1 (96% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGATC416230.2993106212592015Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTA381100.2740486696342928Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGA366640.2636504965487198Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT364090.26181679382616024No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG252820.18180263620294387Illumina Single End PCR Primer 1 (100% over 32bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGATC226020.1625307801383964Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGT215810.1551887782570893Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT183370.1318612032297042Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTAT168760.12135516527809827Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT160580.11547293458376998Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG151980.1092886822645495Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTA145350.10452105518589468Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)