FastQCFastQC Report
Fri 10 May 2024
EGAF00007947662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4456526
Total Bases672.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1064062.3876445464471656Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA984872.2099500821940676No Hit
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT72250.16212179621525824Illumina PCR Primer Index 2 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)