FastQCFastQC Report
Thu 9 May 2024
EGAF00007947663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4456526
Total Bases672.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1095952.459202526811243Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA763181.7124998260977273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72970.16373740442667672No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65610.14722229826550995No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA59190.13281645838036174Illumina Single End PCR Primer 1 (100% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)