FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10188782
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG180876517.752514481122475Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2750102.699145000845047No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA491760.482648465734177No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT262920.25804850864411466Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG226420.22222479585881807Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT215960.2119586030989769No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG188770.18527239075288882Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTGTGTGTAGATC176220.17295492238424573Illumina Single End PCR Primer 1 (97% over 39bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT157530.15461121849500756Illumina Single End PCR Primer 1 (100% over 31bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA124100.12180062347000849No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC115230.11309497052738983No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTGT113690.11158350428932527Illumina Single End PCR Primer 1 (97% over 39bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG113650.11154424542599892Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT112450.11036647952620833Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT108230.10622466944527816Illumina Single End PCR Primer 1 (100% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108160.10615596643445703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)