FastQCFastQC Report
Fri 10 May 2024
EGAF00007947668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165408
Total Bases779.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290222.4978084983799924No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1226862.3751463582354Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT71140.13772387389340784Illumina PCR Primer Index 2 (96% over 31bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA56880.11011714853889566No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)