FastQCFastQC Report
Thu 9 May 2024
EGAF00007947669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165408
Total Bases779.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1248412.416866199146321Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1218272.3585165005358726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260050.5034452263983794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108200.2094703845272242No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72960.14124731289377335No Hit
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65180.12618557914495815No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC58860.11395034041841419No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA58230.11273068845674919No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)