FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10665770
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG169323815.875440779240504Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA555900.521200063380328No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG301200.2823987391440093Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCT286160.2682975537631132TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAA273370.25630592071646024TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA224910.21087085133094002TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACT223340.20939885259104596TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT220490.20672675296767135Illumina PCR Primer Index 2 (96% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAT214520.201129407440813TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG210980.1978103784349372Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGC204210.19146296985590353TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATA194090.18197467224588568TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCT189100.17729615395794207TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATT188390.17663047299913648TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTT187420.1757210215483739TruSeq Adapter, Index 1 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCA181220.16990803289401515TruSeq Adapter, Index 1 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTA172220.16146982355704276TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAC170750.16009158269867063TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTT169690.1590977491545383TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAT167130.15669754738757727TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAT165250.15493489921496525TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTA164350.15409107828126803TruSeq Adapter, Index 1 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT163820.15359416150920185Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT162470.15232843010865602TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAC162350.15221592065082973TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAT161770.15167212493800258TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGT161360.15128771762376275TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATC153830.1442277491451625TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATT152650.1431214061432039TruSeq Adapter, Index 1 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA151320.14187442631896244TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACA150380.14099310223265643TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTA149570.14023366339232893TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACC149390.14006489920558945TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGCT149340.1400180202648285TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAA148250.13899605935623963TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATA148140.13889292568656553TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAA147940.1387054099235217TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAA144020.1350301009678626TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTC143490.13453318419579646TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATG141160.13234862555633584TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACCT138920.13024844901024493TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTC138120.12949838595806962TruSeq Adapter, Index 1 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC136990.12843892189687195Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACA134920.1264981337493683TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACT127160.11922252214326767TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATG126900.1189787516513107TruSeq Adapter, Index 1 (97% over 37bp)
GGACGTAGGAGGCTGCGATAAGCCTCGGGGAGCTGCCAACCGAGCTTTGA124050.1163066520279361No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAC123840.11610976047674007TruSeq Adapter, Index 1 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT121860.11425335442260615TruSeq Adapter, Index 1 (97% over 37bp)
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA121620.11402833550695356No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTG118890.11146874534140527TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAC118720.111309356942818TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCA117340.11001549817781556TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAG115130.10794344899618125TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAGC114790.10762467219900672TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGTT110880.10395873903149984TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGA108460.10168979829866948TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG108170.10141790044225593Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACCA106820.10015216904171007TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)