FastQCFastQC Report
Wed 8 May 2024
EGAF00007947673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10665770
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG182470217.108019392880212Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1073441.0064346034088492No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA515720.4835281465848223No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGT309900.2905556748364159Illumina Single End Sequencing Primer (100% over 33bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA235980.22124984881541607Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT205150.19234429394220953Illumina Single End PCR Primer 1 (96% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGTAGATC203330.19063790049851068Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG179800.16857667097640397Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT170750.16009158269867063Illumina Single End PCR Primer 1 (100% over 31bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA141050.1322454918866617Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG135450.12699505052143445Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT113050.1059932850605254No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG110320.1034336948949771Illumina Single End PCR Primer 1 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)