FastQCFastQC Report
Thu 9 May 2024
EGAF00007947676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3657911
Total Bases552.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG39049210.675273400583011Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331130.9052434572629022No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAA74840.204597651501089TruSeq Adapter, Index 3 (96% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAT68550.18740204450026257TruSeq Adapter, Index 3 (96% over 32bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACG63610.17389706857274548TruSeq Adapter, Index 3 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG59120.16162230300299815Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAG52560.14368856978750985TruSeq Adapter, Index 3 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT47260.12919942557377695Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAG46650.12753180708880013TruSeq Adapter, Index 3 (96% over 32bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC36700.10033048917811285Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)