FastQCFastQC Report
Wed 8 May 2024
EGAF00007947678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6014163
Total Bases908.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1328712.2093016102157526Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA389330.6473552512627277No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA72230.1200998376665215No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)