FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9328340
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG118038512.653751900123709Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG602200.6455596601324566No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA508400.5450058638514462No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG149410.16016783264760934Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT134200.14386268081995296Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG129640.1389743512779337Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTCGCAGTGT124670.13364650087796973Illumina Single End PCR Primer 1 (97% over 38bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG113000.12113623645793356Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG96350.10328740161700796Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTCGCA93790.10054307626008487Illumina Single End PCR Primer 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)