FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44851354
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG13571113.0257971699137554Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5244741.1693604612248718No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACA3598580.8023347522574236TruSeq Adapter, Index 15 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACAC2487640.554641003703032TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTT2286410.5097750226225054TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTA2252780.5022769212273949TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAT2131930.4753323612036328TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTC2002930.44657068769874814TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACT1978950.44122413784877035TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAC1803070.40201016005001766TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGT1731620.38607976026766105TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTG1714140.3821824420283945TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGC1704310.37999075791558046TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAA1679910.3745505654076798TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACA1668760.37206457579853663TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCT1654320.3688450520356643TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCA1434990.31994351831608026TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACAG1422550.31716991197188826TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACG1366780.30473550475198585TruSeq Adapter, Index 15 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGA1282310.28590218257401995TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCC1192430.2658626537785236TruSeq Adapter, Index 15 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACC1147850.2559231545161379TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACA1052780.23472647001916597TruSeq Adapter, Index 13 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGA1051390.23441655741318312TruSeq Adapter, Index 15 (97% over 36bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAA1007480.22462644048605535TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGG973640.21708151776198328TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACT861890.192165881993217TruSeq Adapter, Index 13 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCG848090.18908905180432234TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTCT842210.18777805459340202TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACC691530.15418263627002207TruSeq Adapter, Index 13 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTGC653230.14564331770229277TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCTC650770.14509483927731592TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTTT647950.14446609571697658TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACGCT630080.14048182358106737TruSeq Adapter, Index 15 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAAC584500.13031936560934146TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACACT525070.11706892951325394TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCCT497280.11087290698069004TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACG496280.11064994827134984TruSeq Adapter, Index 13 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTTC495370.11044705584585027TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTCA493190.10996100585948865TruSeq Adapter, Index 15 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACCTT470820.10497341953154858TruSeq Adapter, Index 15 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA466490.10400800832010557No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACTAC465140.1037070140624963TruSeq Adapter, Index 15 (97% over 36bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA456090.10168923774296758Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)