FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44851354
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG15331253.418235712571799Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6095331.3590069098025448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3850300.8584579185725363No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATTCC1508400.3363109171687437Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACAT843820.18813701811543973Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATT695040.15496522133980614Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATTCCG689020.15362300990957822Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATTCCGT644170.14362331179567064Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACATTCCGTG579940.1293026738947502Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)