FastQCFastQC Report
Wed 8 May 2024
EGAF00007947688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729278
Total Bases261.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG79519145.98398869354725Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA355792.057448252970315No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT100710.5823817801417701Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG93460.5404567686629912Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC76980.4451568805015735Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGT76510.44243898320570785TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTT63860.3692870666254934TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTC58000.335400091830232TruSeq Adapter, Index 6 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGA53740.3107655333613219TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAT53720.3106498781572425TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTA52630.30434666953491574TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCT50410.2915089418821034TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAC48090.27809293820889414TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCT46230.2673370042295108TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACT43500.251550068872674TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGA41370.23923278963821895TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGC41130.23784492718926628TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGC40790.23587878871991663TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCC40680.23524268509748TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAA40250.2327560982097731TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCA39490.22836120045475627TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC39020.22564330315889058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG38700.22379281989362035Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTT37270.21552347280194392TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCT36940.213615161934634TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG36730.2124007822918004Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGT33850.1957464329043682TruSeq Adapter, Index 6 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACA33800.19545729489416971TruSeq Adapter, Index 6 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGG33400.1931441908125819TruSeq Adapter, Index 9 (96% over 33bp)
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33100.19140936275139103No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTG32730.18926974147592232TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACC32160.1859735681596597TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG31370.18140518759852378Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAC31360.18134735999648408TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG30750.1778198762720627Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCC30140.17429239254764126TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCA29270.1692613911701878TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAATAGCACACGTCTGAACTCCAG28970.16752656310899694Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCT28600.1653869418335282TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAT27890.16128118208870987TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGT27100.15671280152757394TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACA27070.15653931872145485TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCA25800.14919521326241356TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTA25770.1490217304562945TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATC25650.14832779923181813TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGGCTGAACTCCAG25390.14682428157878608Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCC25090.1450894535175952TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAAC24700.14283417703804707TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAGC24430.1412728317829753TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCAAG24020.1389019000993478Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGTC23770.13745621004835543TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACAGATCGGAAGAGCACACGTCTGAACTCCAGT23760.13739838244631575Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGA23720.13716707203815698TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTC23590.13641531321164094TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAG22350.12924469055871873TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAG22150.12808813851792483TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCA22050.12750986249752788TruSeq Adapter, Index 6 (97% over 38bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG22040.12745203489548818Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGTT21500.12432934438534463TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAA21490.12427151678330495TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGAC21350.1234619303547492TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG21060.12178492989559804Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCC20820.12039706744664536TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAC20780.12016575703848659TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGATCACACGTCTGAACTCCAG20420.11808396336505755Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGC20400.11796830816097816TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTA20330.1175635149467003TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCG20250.11710089413038273TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAAT20120.11634913530386672TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGA20080.11611782489570792TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCCTACGAGATCGGAAGAGCACACGTCTGAACTCCAG19870.11490344525287431Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT19730.11409385882431859Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAA19650.11363123800800103TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTG19190.11097116831417504TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATT19040.11010375428357963TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGGC18440.1066340981611979TruSeq Adapter, Index 6 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAG18400.10640278775303914TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACCCGTCTGAACTCCAG17870.1033379248449353Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATG17360.10038871714091083TruSeq Adapter, Index 6 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)