FastQCFastQC Report
Wed 8 May 2024
EGAF00007947689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729278
Total Bases261.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG78666745.49106621376089Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA321521.8592730607802792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157490.9107269045231594No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG101740.5883380231518588Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT101460.5867188502947472No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT99020.5726089153970617Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG84300.48748668519463034Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG73070.4225462881040527Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT70970.4104024916757167Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT65440.37842382774776523No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTATGGGTGT54320.31411953427962425Illumina Single End PCR Primer 1 (97% over 34bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47760.27618462734158417No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTGGGAAAGAGT38190.2208436121895959Illumina Single End PCR Primer 1 (95% over 23bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG36820.21292123071015764Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT32890.19019498310855745Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT30930.17886077310877718No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGACGAGCGTCGTGTAGGGAAAGAG26320.15220224856847772Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAACGAG23120.13369741591577528Illumina Single End PCR Primer 1 (96% over 30bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.1305747254056317No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTCGGGAAAGAG21660.12525458601797976Illumina Single End PCR Primer 1 (96% over 30bp)
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21030.12161144708947896No Hit
CGTATGCCGTCTTCAGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG20790.12022358464052628Illumina Single End PCR Primer 1 (100% over 30bp)
GTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT20750.1199922742323675Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGAGGGAAAGAGT19760.11426734163043767No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGTAGGGAAAGAGT19590.11328427239576287No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG19500.11276382397740559Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTA18780.10860023663054755Illumina Single End PCR Primer 1 (97% over 34bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.10125613117150625No Hit
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.10102482076334747No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)