FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39355759
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG26855536.823786577207162Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCC4469771.1357346710045664TruSeq Adapter, Index 12 (97% over 34bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3905620.9923884329101619No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAG3862620.9814624588995985TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCA3858500.9804155981339352TruSeq Adapter, Index 12 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTA3519080.8941715493277616TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAA3352250.8517813110909638TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAT3266400.8299674769326644TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCT3025490.7687540723074353TruSeq Adapter, Index 12 (97% over 34bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGC2400530.6099564742227434TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTC2347360.5964463803124722TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAC2279840.579290060191699TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTT2165160.5501507415979451TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCG2008040.5102277407481838TruSeq Adapter, Index 12 (97% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCA1772370.4503457803977304TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCA1711640.4349147478009508TruSeq Adapter, Index 22 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACA1705340.4333139655621938TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCT1602310.407134823648046TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGT1438800.36558817224183127TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCT1390950.3534298499998437TruSeq Adapter, Index 22 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTA1290920.3280129853422469TruSeq Adapter, Index 22 (97% over 37bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGC1227180.31181713456472787TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGC1206850.306651435689501TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCC1200180.3049566392557694TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCT1185240.3011604985181457TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAG1172760.29798942513089377TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCC1107790.28148104067818897TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTG1035980.2632346640805479TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGA1030580.2618625650187562TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCT903950.229686842019741TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTT872770.22176424039998824TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCA850440.21609035668706073TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACT820450.20847012504573983TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCG817680.20776628904552444TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTC803640.20419883148486603TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAC791880.20121070463918633TruSeq Adapter, Index 22 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCG791550.20112685414096576TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCT781280.19851732499937302TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATC774570.19681236486888742TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCC738420.1876269239274486TruSeq Adapter, Index 22 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG718250.18250187983923777Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTGC712460.18103068473409445TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTC696740.17703635190976752TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAT696120.17687881461008031TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTT690740.17551179739666564TruSeq Adapter, Index 22 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGG654040.16618660562485912TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATT649710.16508638545123724TruSeq Adapter, Index 22 (97% over 38bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAC643440.1634932259850458TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTA640020.16262422991257772TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCC613210.1558120121632008TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTAA608550.15462794149135836TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATA600540.15259266121636733TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAA600080.1524757787036962TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGTC594940.15116974367080557TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTCG587490.14927675515037075TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAC582500.14800883398030767TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAT580010.1473761438573704TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACA553770.14070875878673816TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGTT551940.1402437696602421TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCACC536820.13640189228722535TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCA531670.13509331633014626TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCCA528540.13429800705914474TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGCC527030.1339143275066808TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTTG526430.1337618720553706TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCGGC523570.1330351677374587TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCGC498780.12673621667415944TruSeq Adapter, Index 22 (97% over 38bp)
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG495970.12602221697719004No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCTA495550.12591549816127293TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCTGT491600.12491183310681417TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAT480930.12220066699768133TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCGT454610.11551295453354107TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAGC452540.11498698322652091TruSeq Adapter, Index 22 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCCAA449640.11425011521185502TruSeq Adapter, Index 22 (97% over 38bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT431780.10971202461118841TruSeq Adapter, Index 12 (100% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCAAC425020.1079943598597603TruSeq Adapter, Index 22 (97% over 38bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC398060.10114402824755583Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA393690.10003364437717999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)