FastQCFastQC Report
Sun 5 May 2024
EGAF00007947691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39355759
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG29850597.584808617208984Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9970142.533336988876266No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4887341.2418360423438917No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG868590.22070213408919392No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCA815560.20722761311756177Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCA706480.17951121206936957Illumina Single End Sequencing Primer (100% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCAGTGT502450.12766873585134006Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCAGTG501440.12741210250830126Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCAG449300.11416372378944592Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG441760.11224786695131456Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCAC428440.10886335593222837Illumina Single End Sequencing Primer (100% over 33bp)
TCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCACCAG421030.10698053110854754Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)