FastQCFastQC Report
Wed 8 May 2024
EGAF00007947692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071204
Total Bases312.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG23271611.235783631163324Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1326906.406418682080568No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137120.6620303939158093No Hit
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101050.4878804791802256No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79110.38195175366598366No Hit
CTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77990.3765442708685383No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72050.34786529960351564No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67690.32681474157060336No Hit
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63790.3079851139723562No Hit
ATTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63740.3077437084903274No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG57060.2754919360912783Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55060.2658357168101259No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAA54990.26549774913528557TruSeq Adapter, Index 1 (96% over 32bp)
CTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50350.24309532040301196No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT46530.22465194157601084Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.21335416501706253No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAT42180.20364966463950435TruSeq Adapter, Index 1 (96% over 32bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC39700.19167595273087537Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36130.1744396013140183No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAC36130.1744396013140183TruSeq Adapter, Index 1 (96% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAG34210.165169630804112TruSeq Adapter, Index 1 (96% over 32bp)
CTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34040.16434885216521405No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.15696184441513245No Hit
AGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29340.1416567368545059No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA27200.1313245822236728No Hit
TTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26950.13011755481352874No Hit
TCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.12417897995562002No Hit
TTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25030.12084758430362243No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA25010.1207510221108109No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24080.11626088014507505No Hit
CCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23440.11317088997510627No Hit
CATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21920.10583216332143044No Hit
GTTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21640.1044802926220691No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21290.10279045424786742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)