FastQCFastQC Report
Fri 10 May 2024
EGAF00007947694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11987470
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG253890.21179615048046002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA225550.18815479830189358No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG208920.17428197943352516Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC139760.1165884043922529No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC122680.10234019355210065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)