FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11987470
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG331140.27623843897002454No Hit
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGGACTGGTGTAG283980.23689736032707484Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG242710.20246974549258515Illumina Single End PCR Primer 1 (100% over 30bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT208940.17429866352116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162550.13559992225215162No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157760.13160408326360776No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA131280.10951435123508131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)